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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Treinta y Tres. |
Fecha : |
27/09/2017 |
Actualizado : |
25/10/2019 |
Tipo de producción científica : |
Documentos |
Autor : |
(INIA) INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA, URUGUAY; (INASE) INSTITUTO NACIONAL DE SEMILLAS, URUGUAY. |
Título : |
Resultados experimentales de la evaluación nacional de cultivares de trigo ciclo largo: período 2016. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
La Estanzuela (UY): INASE ; INIA, 2017. |
Páginas : |
31 p. |
Idioma : |
Español |
Notas : |
Editado por el Equipo de Evaluación de Cultivares - Impreso por Unidad de Comunicación y Transferencia de Tecnología INIA La Estanzuela.
Convenio INASE-INIA. |
Contenido : |
EQUIPOS DE TRABAJO
INIA
Evaluación de Cultivares
Ing. Agr. (PhD.) Marina Castro
Evaluación de Cultivares
Ing. Agr. Santiago Manasliski
Ensayos regionales Young
Téc. Sist. Int. Gan. Máximo Vera
Asistente de Investigación
Beatriz Castro
Valeria Cardozo
Asistentes de Información y Proc. de datos
Protección Vegetal
Ing. Agr. (Ph.D.) Silvia Pereyra (Fitopatología)
Lic. Biol. (Ph.D.) Gustavo Azzimonti (Fitopatología)
Ing. Agr. (Ph.D.) Silvia Germán (Mej. por resistencia)
Tec. Agrop. Richard García (Mej. por resistencia)
Tec. Lech. Néstor González (Fitopatología)
Unidad de Comunicación y Transferencia de Tecnología
Ing. Agr. (M.Sc.) Ernesto Restaino
Amado Vergara (Asistente de UCTT)
SOCIEDAD RURAL DE RÍO NEGRO
Ing. Agr. (MBA) Donald Chalkling
Téc. Agrop. Santiago Salaberry
Martha Roth
INASE
Área Evaluación y Registro de Cultivares
Ing. Agr. (M.Sc.) Gerardo Camps
Gerente
Ing. Agr. (M.Sc.) Virginia Olivieri
Ing. Agr. (M.Sc.) Sebastián Moure
Ing. Agr. (M.Sc.) Federico Boschi
Área Laboratorio de Calidad de Semillas
Ph.D. Vanessa Sosa
Gerente
Ing. Agr. Teresita Farrás
Analista Fabián Makowski
Analista Mónica Rojas
Analista Laura Tellechea
Área Administración
Daniel Almeida.
TABLA DE CONTENIDO.I. PRESENTACIÓN II. CARACTERIZACIÓN DE LA ZAFRA 2016. III. EVALUACION DE CULTIVARES DE TRIGO CICLO LARGO.
1. INTRODUCCION 2. OBJETIVO.3. MATERIALES Y METODOS. 3.1 Ensayos conducidos en La Estanzuela y Young. 3.2 Ensayos conducidos en Dolores.
4. RESULTADOS EXPERIMENTALES ? Ensayos sin fungicidas.4.1 Rendimiento de grano.4.2 Comportamiento sanitario.
4.2.1 Comportamiento sanitario en colecciones.4.3 Características agronómicas.4.4 Calidad de grano.
5. RESULTADOS EXPERIMENTALES ? Ensayos con fungicidas.5.1 Rendimiento de grano.5.2 Características agronómicas.
5.3 Calidad de grano.IV. CONDICIONES CLIMATICAS. MenosEQUIPOS DE TRABAJO
INIA
Evaluación de Cultivares
Ing. Agr. (PhD.) Marina Castro
Evaluación de Cultivares
Ing. Agr. Santiago Manasliski
Ensayos regionales Young
Téc. Sist. Int. Gan. Máximo Vera
Asistente de Investigación
Beatriz Castro
Valeria Cardozo
Asistentes de Información y Proc. de datos
Protección Vegetal
Ing. Agr. (Ph.D.) Silvia Pereyra (Fitopatología)
Lic. Biol. (Ph.D.) Gustavo Azzimonti (Fitopatología)
Ing. Agr. (Ph.D.) Silvia Germán (Mej. por resistencia)
Tec. Agrop. Richard García (Mej. por resistencia)
Tec. Lech. Néstor González (Fitopatología)
Unidad de Comunicación y Transferencia de Tecnología
Ing. Agr. (M.Sc.) Ernesto Restaino
Amado Vergara (Asistente de UCTT)
SOCIEDAD RURAL DE RÍO NEGRO
Ing. Agr. (MBA) Donald Chalkling
Téc. Agrop. Santiago Salaberry
Martha Roth
INASE
Área Evaluación y Registro de Cultivares
Ing. Agr. (M.Sc.) Gerardo Camps
Gerente
Ing. Agr. (M.Sc.) Virginia Olivieri
Ing. Agr. (M.Sc.) Sebastián Moure
Ing. Agr. (M.Sc.) Federico Boschi
Área Laboratorio de Calidad de Semillas
Ph.D. Vanessa Sosa
Gerente
Ing. Agr. Teresita Farrás
Analista Fabián Makowski
Analista Mónica Rojas
Analista Laura Tellechea
Área Administración
Daniel Almeida.
TABLA DE CONTENIDO.I. PRESENTACIÓN II. CARACTERIZACIÓN DE LA ZAFRA 2016. III. EVALUACION DE CULTIVARES DE TRIGO CICLO LARGO.
1. INTRODUCCION 2. OBJETIVO.3. MATERIALES Y METODOS. 3.1 Ensayos conducidos en La Estanzuela y Young. 3.2 Ensayos conducidos en Dolores.
4. RESULTADOS EXPERIMENTALES ? Ensayos sin fungicidas.4... Presentar Todo |
Thesagro : |
EVALUACION DE CULTIVARES; TRIGO. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9394/1/PubTrigoLargoPeriodo2016.pdf
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Marc : |
LEADER 02592nam a2200169 a 4500 001 1057613 005 2019-10-25 008 2017 bl uuuu u0uu1 u #d 100 1 $a(INIA) INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA, URUGUAY 245 $aResultados experimentales de la evaluación nacional de cultivares de trigo ciclo largo$bperíodo 2016.$h[electronic resource] 260 $aLa Estanzuela (UY): INASE ; INIA$c2017 300 $a31 p. 500 $aEditado por el Equipo de Evaluación de Cultivares - Impreso por Unidad de Comunicación y Transferencia de Tecnología INIA La Estanzuela. Convenio INASE-INIA. 520 $aEQUIPOS DE TRABAJO INIA Evaluación de Cultivares Ing. Agr. (PhD.) Marina Castro Evaluación de Cultivares Ing. Agr. Santiago Manasliski Ensayos regionales Young Téc. Sist. Int. Gan. Máximo Vera Asistente de Investigación Beatriz Castro Valeria Cardozo Asistentes de Información y Proc. de datos Protección Vegetal Ing. Agr. (Ph.D.) Silvia Pereyra (Fitopatología) Lic. Biol. (Ph.D.) Gustavo Azzimonti (Fitopatología) Ing. Agr. (Ph.D.) Silvia Germán (Mej. por resistencia) Tec. Agrop. Richard García (Mej. por resistencia) Tec. Lech. Néstor González (Fitopatología) Unidad de Comunicación y Transferencia de Tecnología Ing. Agr. (M.Sc.) Ernesto Restaino Amado Vergara (Asistente de UCTT) SOCIEDAD RURAL DE RÍO NEGRO Ing. Agr. (MBA) Donald Chalkling Téc. Agrop. Santiago Salaberry Martha Roth INASE Área Evaluación y Registro de Cultivares Ing. Agr. (M.Sc.) Gerardo Camps Gerente Ing. Agr. (M.Sc.) Virginia Olivieri Ing. Agr. (M.Sc.) Sebastián Moure Ing. Agr. (M.Sc.) Federico Boschi Área Laboratorio de Calidad de Semillas Ph.D. Vanessa Sosa Gerente Ing. Agr. Teresita Farrás Analista Fabián Makowski Analista Mónica Rojas Analista Laura Tellechea Área Administración Daniel Almeida. TABLA DE CONTENIDO.I. PRESENTACIÓN II. CARACTERIZACIÓN DE LA ZAFRA 2016. III. EVALUACION DE CULTIVARES DE TRIGO CICLO LARGO. 1. INTRODUCCION 2. OBJETIVO.3. MATERIALES Y METODOS. 3.1 Ensayos conducidos en La Estanzuela y Young. 3.2 Ensayos conducidos en Dolores. 4. RESULTADOS EXPERIMENTALES ? Ensayos sin fungicidas.4.1 Rendimiento de grano.4.2 Comportamiento sanitario. 4.2.1 Comportamiento sanitario en colecciones.4.3 Características agronómicas.4.4 Calidad de grano. 5. RESULTADOS EXPERIMENTALES ? Ensayos con fungicidas.5.1 Rendimiento de grano.5.2 Características agronómicas. 5.3 Calidad de grano.IV. CONDICIONES CLIMATICAS. 650 $aEVALUACION DE CULTIVARES 650 $aTRIGO 700 1 $a(INASE) INSTITUTO NACIONAL DE SEMILLAS, URUGUAY.
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Registro original : |
INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
17/05/2022 |
Actualizado : |
02/12/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
PASSOS, J. R. S.; GUERREIRO, D. D.; OTÁVIO, K. S.; SANTOS-NETO, P. C. DOS; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ-OLIVERA, R.; CRISPO, M.; BEZERRA, M. J. B.; SILVA, R. F.; LIMA, L. F.; FIGUEIREDO, J. R.; BUSTAMANTE-FILHO, I. C.; MENCHACA, A.; MOURA, A. A. |
Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; DENISE D. GUERREIRO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; KAMILA S. OTÁVIO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; P. C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; FEDERICO CUADRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; RICHARD NUÑEZ-OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay; MARIA JÚLIA B. BEZERRA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; RENATO F. SILVA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; LARITZA F. LIMA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; JOSÉ RICARDO FIGUEIREDO, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; IVAN C. BUSTAMANTE-FILHO, aboratório de Biotecnologia da Reprodução Animal, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; ARLINDO A. MOURA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil. |
Título : |
Global proteomic analysis of preimplantational ovine embryos produced in vitro. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Reproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122 |
ISSN : |
0936-6768 |
DOI : |
10.1111/rda.14122 |
Idioma : |
Inglés |
Notas : |
Article history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com |
Contenido : |
ABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated
with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro.
© 2022 Wiley-VCH GmbH. MenosABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational i... Presentar Todo |
Palabras claves : |
Embryo development; In vitro fertilization; Mass spectrometry; Oocyte; Ovine; PLATAFORMA SALUD ANIMAL; Proteins. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
Marc : |
LEADER 04571naa a2200409 a 4500 001 1063149 005 2022-12-02 008 2022 bl uuuu u00u1 u #d 022 $a0936-6768 024 7 $a10.1111/rda.14122$2DOI 100 1 $aPASSOS, J. R. S. 245 $aGlobal proteomic analysis of preimplantational ovine embryos produced in vitro.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com 520 $aABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro. © 2022 Wiley-VCH GmbH. 653 $aEmbryo development 653 $aIn vitro fertilization 653 $aMass spectrometry 653 $aOocyte 653 $aOvine 653 $aPLATAFORMA SALUD ANIMAL 653 $aProteins 700 1 $aGUERREIRO, D. D. 700 1 $aOTÁVIO, K. S. 700 1 $aSANTOS-NETO, P. C. DOS 700 1 $aSOUZA-NEVES, M. 700 1 $aCUADRO, F. 700 1 $aNUÑEZ-OLIVERA, R. 700 1 $aCRISPO, M. 700 1 $aBEZERRA, M. J. B. 700 1 $aSILVA, R. F. 700 1 $aLIMA, L. F. 700 1 $aFIGUEIREDO, J. R. 700 1 $aBUSTAMANTE-FILHO, I. C. 700 1 $aMENCHACA, A. 700 1 $aMOURA, A. A. 773 $tReproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122
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